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Differential Expression Analysis by RNA-Seq Reveals Perturbations in the Platelet mRNA Transcriptome Triggered by
Abdimajid Osman1, Walter E Hitzler2, Adam Ameur3
1Department of Clinical Chemistry, Region Östergötland, Ingång 64, Linköping, Sweden; Department of Clinical and Experimental Medicine, University of Linköping, Linköping, Sweden.
Plos One
|July 15, 2015
Summary
Pathogen reduction (PR) systems like Intercept significantly alter the mRNA transcriptome in human platelets, affecting over 800 genes. This impacts blood product safety and regulatory considerations for PR systems.
Area of Science:
- Biotechnology
- Hematology
- Molecular Biology
Background:
- Platelet concentrates (PCs) are crucial for patients with low platelet counts.
- Pathogen reduction (PR) systems aim to prevent transfusion-transmitted infections by inactivating pathogen nucleic acids.
- PR systems may inadvertently affect platelet nucleic acids, impacting platelet function.
Purpose of the Study:
- To investigate the impact of the Intercept PR system on the mRNA transcriptome of human platelets.
- To compare the effects of Intercept with other PR systems and control platelets.
- To analyze the interplay between microRNA (miRNA) and mRNA expression in stored platelets treated with PR systems.
Main Methods:
- Genome-wide differential expression (DE) RNA sequencing (RNA-Seq) was employed to analyze mRNA levels.
- Analysis included comparison between Intercept-treated platelets, other PR systems, and control platelets.
- Integrated analysis of miRNA and mRNA expression data, including predicted miRNA-mRNA interactions.
Main Results:
- Intercept treatment markedly perturbed the mRNA transcriptome, altering expression levels of over 800 mRNAs (P<0.05).
- Approximately 400 genes exhibited differential expression with fold changes (FC) ≥ 2.
- At p<0.001, 147 genes were deregulated by ≥ 2-fold in Intercept-treated platelets, unlike other groups.
Conclusions:
- The Intercept PR system significantly deregulates the platelet mRNA transcriptome.
- Intercept treatment is associated with reduced levels of mRNA-regulatory miRNAs.
- Findings highlight the need for careful consideration of PR systems targeting nucleic acids for blood product management.
