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Updated: Apr 7, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Ballistic imaging of biological media with collimated illumination and focal plane detection
Abstract:
A simple, affordable method for imaging through biological tissue is investigated. The method consists of (1) imaging with a wavelength that has a relatively small scattering coefficient (1310 nm in this case) and (2) collimated illumination together with (3) focal plane detection to enhance the detection of the ballistic photons relative to the diffusive light. We demonstrate ballistic detection of an object immersed in a 1-cm-thick cuvette filled with 4% Intralipid, which is equivalent to ∼1 to 2 cm of skin tissue. With the same technology, a ballistic image of a 1-mm-wide object in 10-mm-thick chicken breast is also presented.
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