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Published on: December 28, 2016
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Morphokinetic parameters using time-lapse technology and day 5 embryo quality: a prospective cohort study
Ashleigh Storr1, Christos A Venetis, Simon Cooke
1IVF Australia Western Sydney, Level 2, 20-22 Mons Road, Westmead, NSW, 2145, Australia, ashleigh_storr@hotmail.com.
Journal of Assisted Reproduction and Genetics
|July 16, 2015
Summary
Time-lapse imaging identified key embryo development timings that can predict day 5 blastocyst quality. While some parameters show promise, further research is needed for clinical application in IVF embryo selection.
Area of Science:
- Embryology
- Reproductive Medicine
- In Vitro Fertilization (IVF)
Background:
- Assessing day 5 embryo quality is crucial for successful IVF outcomes.
- Traditional methods have limitations in predicting top-quality blastocysts.
- Time-lapse technology offers continuous embryo monitoring.
Purpose of the Study:
- To evaluate time-lapse parameters for predicting day 5 embryo quality.
- To assess the discriminatory capacity of these parameters.
- To identify predictive models for top-quality blastocysts.
Main Methods:
- Prospective cohort study of 380 day 5 blastocysts from 108 patients.
- Utilized time-lapse technology to record key developmental event timings.
- Employed generalized estimating equation and multivariable regression models.
- Assessed discriminatory capacity using receiver operating characteristic (ROC) analyses.
Main Results:
- Identified eight significant predictive parameters for top-quality blastocysts (s3, t6, t7, t8, tM, tSB, tB, tEB).
- Parameter s3 showed the best individual discriminatory capacity before compaction (AUC 0.585).
- Parameter tEB demonstrated the best predictive capacity regardless of embryo stage (AUC 0.727).
- A composite model (s3, t8, tEB) slightly improved prediction (AUC 0.748).
Conclusions:
- Morphokinetic parameters identified offer insights into predictive markers for top-quality blastocysts.
- Current parameters have limited clinical value for embryo selection.
- Further validation is required before integrating time-lapse parameters into routine IVF practice.
