Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Determination of Crystal Structures

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Early administration of renin-angiotensin system inhibitors improves survival and cardiac remodeling in heart failure with preserved ejection fraction.

PloS one·2026

Same author

Effect of Dietary Sodium Nitrite Supplementation on Spontaneous Intracerebral Hemorrhage in Stroke-Prone Spontaneously Hypertensive Rats.

Preventive nutrition and food science·2026

Same author

A study of the clinical usefulness of the eMEMO®.

Journal of arrhythmia·2025

Same author

Structures and mechanisms of actin ATP hydrolysis.

Proceedings of the National Academy of Sciences of the United States of America·2022

Same author

Multiple Late Complications After Takeuchi Repair of Anomalous Left Coronary Artery From the Pulmonary Artery.

JACC. Case reports·2021

Same author

Usefulness of cardiac fusion imaging with computed tomography and Doppler echocardiography in the assessment of conduit stenosis in complex adult congenital heart disease.

Journal of cardiology·2021

Same journal

Future Directions in Biotechnological and Pharmacological Applications of CAIs.

Sub-cellular biochemistry·2026

Same journal

Industrial and Environmental Applications of Carbonic Anhydrases.

Sub-cellular biochemistry·2026

Same journal

Applications of Carbonic Anhydrase Inhibitors in Arthritis, Neuropathic Pain, Acute Mountain Sickness, and Cerebral Ischemia.

Sub-cellular biochemistry·2026

Same journal

Applications of Carbonic Anhydrase Inhibitors in Neurological Disorders, Mechanisms and Therapeutic Potential.

Sub-cellular biochemistry·2026

Same journal

Carbonic Anhydrase Inhibitors in Oncology.

Sub-cellular biochemistry·2026

Same journal

Therapeutic Applications of Carbonic Anhydrase Inhibitors in Ophthalmology.

Sub-cellular biochemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 7, 2026

$High Pressure Single Crystal Diffraction at PX^2$

High Pressure Single Crystal Diffraction at PX^2

Published on: January 16, 2017

High Pressure Macromolecular Crystallography.

Nobuhisa Watanabe¹

¹Synchrotron Radiation Research Center, Nagoya University, Furo-cho Chikusa-ku, Nagoya, 4648603, Japan, nobuhisa@nagoya-u.jp.

Sub-Cellular Biochemistry

|July 16, 2015

Summary

This summary is machine-generated.

High-pressure macromolecular crystallography (HPMX) uses diamond anvil cells (DAC) to study biomacromolecular hydration. This technique reveals detailed water structures under extreme pressures up to 1 GPa.

More Related Videos

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Published on: September 2, 2012

Related Experiment Videos

Last Updated: Apr 7, 2026

$High Pressure Single Crystal Diffraction at PX^2$

High Pressure Single Crystal Diffraction at PX^2

Published on: January 16, 2017

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Published on: September 2, 2012

Area of Science:

Biochemistry
Structural Biology
Biophysics

Background:

X-ray crystallography is vital for high-resolution biomacromolecular structure determination.
Direct observation of hydration water structure is a key advantage of crystallography.
Advancements in high-pressure macromolecular crystallography (HPMX) have enabled studies at extreme pressures.

Purpose of the Study:

To describe the usage of diamond anvil cells (DAC) in HPMX.
To present an HPMX study on the hydration structure of 3-isopropylmalate dehydrogenase (IPMDH).
To highlight the growing number of protein structures determined by HPMX.

Main Methods:

High-pressure macromolecular crystallography (HPMX).
Diamond Anvil Cell (DAC) for generating pressures up to 1 GPa.
Synchrotron radiation with shorter wavelengths.

Main Results:

Demonstration of DAC utility in HPMX.
Detailed analysis of IPMDH hydration structure under high pressure.
Successful structure determination of proteins at pressures from hundreds of MPa to 1 GPa.

Conclusions:

HPMX is an expanding field for structural biology.
DAC technology is crucial for achieving high pressures in macromolecular crystallography.
Understanding hydration structure under pressure provides insights into protein stability and function.