Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasminogen interactions with immobilized fibrinogen.

B Adelman1, P Quynn

  • 1Department of Medicine, Medical College of Virginia, Richmond.

Thrombosis and Haemostasis
|December 29, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of CD40 ligand (CD154) in the treatment of factor VIII inhibitors.

Haematologica·2001
Same author

Effect of transient abrupt vessel closure during otherwise successful angioplasty for unstable angina on clinical outcome at six months. Hirulog Angioplasty Study Investigators.

Journal of the American College of Cardiology·1999
Same author

Effect of direct thrombin inhibition with Bivalirudin (Hirulog) on restenosis after coronary angioplasty.

The American journal of cardiology·1998
Same author

Randomized, double-blind comparison of hirulog versus heparin in patients receiving streptokinase and aspirin for acute myocardial infarction (HERO). Hirulog Early Reperfusion/Occlusion (HERO) Trial Investigators.

Circulation·1997
Same author

Treatment with bivalirudin (Hirulog) as compared with heparin during coronary angioplasty for unstable or postinfarction angina. Hirulog Angioplasty Study Investigators.

The New England journal of medicine·1995
Same author

Aurintricarboxylic acid prevents acute rethrombosis in a canine model of arterial thrombosis.

Arteriosclerosis, thrombosis, and vascular biology·1995
Same journal

Discontinuation of Oral Anticoagulation After Successful Catheter Ablation for Atrial Fibrillation: Meta-analysis of Randomized Clinical Trials.

Thrombosis and haemostasis·2026
Same journal

Novel/Recurrent Variants in Pakistani Glanzmann Thrombasthenia and Glanzmann-like bleeding diathesis: Insights from NGS Analysis.

Thrombosis and haemostasis·2026
Same journal

Anti-TFPI Single-Domain Antibodies: Novel Rebalancing Therapies for Hemophilia and Other Rare Bleeding Disorders.

Thrombosis and haemostasis·2026
Same journal

The Interaction of Fibrin with Endothelial Cell Receptor N-Cadherin Promotes Fibrin-Dependent Angiogenesis.

Thrombosis and haemostasis·2026
Same journal

Antiphospholipid Antibodies and Atrial Fibrillation: Clinical Implications and the TaPL AF Study Design.

Thrombosis and haemostasis·2026
Same journal

Prasugrel vs. Ticagrelor: Can TUXEDO-2 Settle the Debate?

Thrombosis and haemostasis·2026
See all related articles

Immobilized fibrinogen binds both glu- and lys-plasminogen, primarily through plasminogen's lysine-binding regions. This interaction is crucial for understanding plasminogen adhesion to surfaces and cells.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Surface Science

Background:

  • Plasminogen is a key protein in the fibrinolytic system.
  • Fibrinogen is a crucial protein involved in blood clotting and is known to adsorb onto surfaces.
  • Understanding plasminogen-fibrinogen interactions is vital for various physiological and pathological processes.

Purpose of the Study:

  • To investigate the binding characteristics of plasminogen to immobilized fibrinogen.
  • To elucidate the specific regions of plasminogen involved in this binding.
  • To explore factors influencing plasminogen binding to fibrinogen on surfaces.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to quantify plasminogen binding.
  • Immobilized fibrinogen on polystyrene wells served as the substrate.

Related Experiment Videos

  • Inhibition studies with epsilon aminocaproic acid and fibrinogen fragments were performed.

    • Main Results:

    • Both glu-plasminogen and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent manner.
    • Lys-plasminogen showed higher binding affinity than glu-plasminogen at equal concentrations.
    • Binding was inhibited by epsilon aminocaproic acid, confirming mediation via lysine-binding regions.
    • Fibrinogen fragments, but not excess soluble fibrinogen, competed for binding.
    • Thrombin treatment of fibrinogen slightly increased plasminogen binding.

    Conclusions:

    • Immobilized fibrinogen effectively binds both forms of plasminogen.
    • Plasminogen binding is primarily mediated by its lysine-binding regions interacting with fibrinogen.
    • These findings suggest a mechanism for plasminogen adhesion to fibrinogen-coated surfaces and cells like platelets.