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Vitamin K-dependent carboxylase: partial purification of the enzyme by antibody affinity techniques
M C Harbeck1, A Y Cheung, J W Suttie
1Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Thrombosis Research
|October 15, 1989
Abstract:
The vitamin K-dependent carboxylase activity of bovine liver microsomes has been purified 500-fold by adsorption to an antiprothrombin column and elution with a dodeca peptide which competes with a prothrombin precursor enzyme recognition site. The purified enzyme is devoid of bound precursors, and has the same ratio of vitamin K epoxidase activity to carboxylase activity as the crude microsomal preparation.