Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Benchmarking DNA extraction protocols across use cases for culture-independent Nanopore metagenomics.

Microbial genomics·2026
Same author

Toward scalable species descriptions for dark taxa: the role of molecular data.

ZooKeys·2026
Same author

Constructing a lower-bound estimate of the global number of insect species on a hyperdiverse empirical foundation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

How can biological databases support the new UN mechanism for benefit-sharing from digital sequence information?

Scientific data·2026
Same author

MNBC-ME categorizes viral and plasmid sequences within metagenomes and identifies putative species or plasmid host.

BMC bioinformatics·2026
Same author

Barcoding Megadiversity: An Arthropod Database from Sites in the Neotropical Eastern Pacific Bioregion.

Scientific data·2026

Related Experiment Video

Updated: Apr 7, 2026

A Rapid Method to Confine and Safely Handle Bees in the Field
03:44

A Rapid Method to Confine and Safely Handle Bees in the Field

Published on: August 23, 2024

1.9K

Untangling taxonomy: a DNA barcode reference library for Canadian spiders.

Gergin A Blagoev1, Jeremy R deWaard1, Sujeevan Ratnasingham1

  • 1Biodiversity Institute of Ontario, University of Guelph, Guelph, ON, Canada.

Molecular Ecology Resources
|July 16, 2015
PubMed
Summary

DNA barcoding of Canadian spiders created a reference library for 1018 species. This research identified new species and range extensions, potentially increasing Canada's spider count by 50%.

Keywords:
AraneaeBarcode Index NumbersDNA barcodingoperational taxonomic unitsspecies identificationspiders

More Related Videos

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
10:23

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

Published on: July 11, 2025

758
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

11.2K

Related Experiment Videos

Last Updated: Apr 7, 2026

A Rapid Method to Confine and Safely Handle Bees in the Field
03:44

A Rapid Method to Confine and Safely Handle Bees in the Field

Published on: August 23, 2024

1.9K
A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
10:23

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

Published on: July 11, 2025

758
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

11.2K

Area of Science:

  • Arachnology
  • Genomics
  • Biodiversity Research

Background:

  • Canada hosts approximately 1460 spider species, representing 3% of the global diversity.
  • Establishing comprehensive reference data is crucial for accurate species identification and biodiversity assessment.

Purpose of the Study:

  • To construct a DNA barcode reference library for Canadian spiders.
  • To assess species diversity, identify new species, and delineate species boundaries using DNA barcoding.

Main Methods:

  • Analysis of over 30,000 spider specimens from Canada.
  • DNA extraction, sequencing, and analysis to generate a reference library.
  • Assignment of sequences to Barcode Index Numbers (BINs) for taxonomic comparison.

Main Results:

  • A library of DNA barcodes for 1018 Canadian spider species was established.
  • DNA barcodes successfully discriminated 98% of the studied species.
  • The study identified 16 species new to science, 52 new to Canada, and range extensions for 426 species.

Conclusions:

  • DNA barcoding is an effective tool for spider species identification and biodiversity assessment in Canada.
  • The findings suggest that the actual number of Canadian spider species may be 30-50% higher than currently recognized, highlighting the presence of cryptic diversity.
  • The established reference library will support future taxonomic and ecological research on Canadian arachnofauna.