Related Experiment Video

Updated: Apr 7, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

11.7K

In Silico Docking to Explicate Interface between Plant-Originated Inhibitors and E6 Oncogenic Protein of Highly

Satish Kumar1, Lingaraja Jena1, Maheswata Sahoo1

  • 1Bioinformatics Centre & Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram 442-102, India.

Genomics & Informatics
|July 16, 2015
PubMed

Insights

Natural compounds show promise in fighting human papillomavirus (HPV) by inhibiting the E6 oncoprotein. This study used structure-based drug design to identify compounds that could restore tumor suppressor p53 protein function.

Area of Science:

  • Oncology
  • Virology
  • Drug Discovery

Background:

  • Human papillomavirus (HPV) infection is a leading cause of cancer mortality in women worldwide.
  • The E6 oncoprotein of high-risk HPV strains, like HPV18, inactivates the tumor suppressor p53 protein, promoting cancer development.
  • Natural compounds have historically been explored for their therapeutic potential in cancer treatment.

Purpose of the Study:

  • To investigate the potential of various natural compounds as inhibitors of the HPV18 E6 oncoprotein.
  • To elucidate the molecular mechanisms by which these natural compounds interact with the E6 oncoprotein.
  • To identify natural compounds capable of restoring the function of the tumor suppressor p53 protein.

Main Methods:

  • Structure-based drug designing approach was employed.
  • Molecular docking analysis was performed to predict interactions between natural inhibitors and the HPV18 E6 oncoprotein.
  • In silico validation of natural compounds was conducted.

Main Results:

  • Docking analysis revealed interactions between several natural inhibitors and the p53 binding site (residues 108-117, CQKPLNPAEK) of the HPV18 E6 oncoprotein.
  • These interactions suggest a mechanism for reinstating normal p53 functioning.
  • The study provided in silico validation for the efficacy of these natural compounds against HPV oncoproteins.

Conclusions:

  • Natural compounds can effectively inhibit the HPV18 E6 oncoprotein.
  • The identified natural compounds have the potential to restore p53 tumor suppressor activity.
  • This research offers a foundation for developing novel anti-cancer therapeutics derived from natural sources against HPV infections.
Keywords:
human papillomavirus 18molecular dockingneoplasmsplant products

More Related Videos

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

13.1K
In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells09:20

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Published on: July 23, 2010

16.4K

Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
32
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

5.7K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.2K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.4K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.7K