Rational, yet simple, design and synthesis of an antifreeze-protein inspired polymer for cellular cryopreservation
Daniel E Mitchell1, Neil R Cameron, Matthew I Gibson
1Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK. m.i.gibson@warwick.ac.uk.
Chemical Communications (Cambridge, England)
|July 16, 2015
Abstract:
Antifreeze (glyco) proteins AF(G)Ps are potent ice recrystallization inhibitors, which is a desirable property to enhance cryopreservation of donor tissue/cells. Here we present the rational synthesis of a new, biomimetic, ice-recrystallization inhibiting polymer derived from a cheap commodity polymer, based on an ampholyte structure. The polymer is used to enhance the cryopreservation of red blood cells, demonstrating a macromolecular solution to tissue storage.
