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Comparing the Affinity of GTPase-binding Proteins using Competition Assays
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Locking GTPases covalently in their functional states.

David Wiegandt1, Sophie Vieweg1, Frank Hofmann1

  • 1Department of Physical Biochemistry, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany.

Nature Communications
|July 17, 2015
PubMed
Summary

Researchers developed novel acryl-bearing nucleotide derivatives to covalently lock GTPases in active or inactive states. This method provides a stable tool for studying GTPase function and intracellular localization mechanisms.

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