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A Highly K(+)-Selective Two-Photon Fluorescent Probe.

Thomas Schwarze1, Janine Riemer1, Sascha Eidner2

  • 1Institut für Chemie, Anorganische Chemie, Universität Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Golm (Germany).

Chemistry (Weinheim an Der Bergstrasse, Germany)
|July 17, 2015
PubMed
Summary
This summary is machine-generated.

A novel two-photon fluorescent probe enables accurate in vitro monitoring of physiological potassium (K+) levels. This highly selective probe demonstrates significant fluorescence enhancement, crucial for biological research and diagnostics.

Keywords:
click chemistryfluorescencefluorescent probespotassiumtwo-photon

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Molecular Imaging

Background:

  • Accurate monitoring of physiological potassium (K+) levels is vital for understanding cellular functions and diagnosing diseases.
  • Existing methods for K+ detection may lack selectivity or sensitivity, necessitating the development of advanced fluorescent probes.

Purpose of the Study:

  • To develop and characterize a highly potassium-selective two-photon fluorescent probe for in vitro monitoring of physiological K+ concentrations.
  • To evaluate the probe's performance, including fluorescence enhancement, selectivity over sodium (Na+) ions, and dissociation constants under various conditions.

Main Methods:

  • Synthesis and characterization of a novel two-photon excited fluorescence (TPEF) probe.
  • In vitro fluorescence measurements to assess K+-induced fluorescence enhancement (FE) under one-photon (OP) and two-photon (TP) excitation.
  • Determination of dissociation constants (Kd) in the presence and absence of competing Na+ ions.
  • Measurement of the TP absorption cross-section (σ2PA).

Main Results:

  • The TPEF probe exhibited a significant fluorescence enhancement (FE ≈ 3x) with 160 mM K+.
  • The probe demonstrated comparable K+-induced FEs irrespective of OP (430 nm) or TP (860 nm) excitation.
  • High K+/Na+ selectivity was confirmed by dissociation constant values (Kd(OP) ≈ 28-38 mM, Kd(TP) ≈ 36-46 mM) in mixed ion solutions.
  • The probe possessed a TP absorption cross-section (σ2PA) of 26 GM at 860 nm.

Conclusions:

  • The developed TPEF probe is highly selective for K+ ions over Na+ ions.
  • Its robust fluorescence response and selectivity make it a suitable tool for in vitro determination of physiological K+ levels (1-100 mM).
  • This probe holds potential for applications in biological research and clinical diagnostics requiring precise K+ ion concentration measurements.