Related Experiment Video
Updated: Apr 7, 2026

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
Published on: December 20, 2010
A New Turn on Fluorescent Probe for Selective Detection of Cysteine/Homocysteine
Puhui Xie1, Guangqin Gao2, Juanjuan Liu2
1College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China. phxie2013@163.com.
Abstract:
A new fluorescent probe (probe 1) was developed for recognition of cysteine (Cys) and homocysteine (Hcy). Probe 1 exhibited a large absorption peak blue-shift (107 nm) as well as enhanced fluorescence responses to Cys/Hcy based on cyclization of thiol containing amino acids with aldehydes, inhibiting the C = N isomerization-induced quenching process by an intramolecular hydrogen bond. The detection mechanism was proved by (1)H NMR and mass spectrometry analysis.

