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Related Experiment Videos

A semi-continuous method for purification of factor IX complex from human plasma.

J P Tharakan1, D M Gee, D B Clark

  • 1American Red Cross, Jerome H. Holland Laboratory for the Biomedical Sciences, Rockville, Md.

Vox Sanguinis
|January 1, 1989
PubMed
Summary

A new semi-continuous method improves factor IX complex preparation from human plasma using ion exchange resins. This approach offers higher factor IX (FIX) recovery and reduced processing times compared to traditional batch methods.

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Area of Science:

  • Biochemistry
  • Process Chemistry

Background:

  • Traditional methods for factor IX complex preparation involve batch adsorption.
  • Batch methods present challenges like high labor costs and process complexity.
  • These methods are often limited by high pressure drops and low flow rates with soft gels in packed columns.

Purpose of the Study:

  • To develop and evaluate a semi-continuous method for factor IX complex preparation.
  • To improve recovery rates and reduce processing times compared to batch adsorption.
  • To investigate the impact of different contact modes on factor IX recovery.

Main Methods:

  • Development of a semi-continuous process using ion exchange resins in a stirred column.
  • Pumping cryo-supernatant plasma through the resin-filled column.

Related Experiment Videos

  • Comparison of lab and pilot scale results with traditional batch adsorption.

    • Main Results:

    • The semi-continuous method achieved higher factor IX (FIX) recoveries.
    • Total process times were decreased compared to batch adsorption.
    • A 15-minute residence time captured 95% of FIX; pilot scale yielded 68% recovery from 550L plasma.

    Conclusions:

    • The semi-continuous method is effective for factor IX complex preparation.
    • Process efficiency is enhanced through higher FIX recovery and shorter durations.
    • Factor IX recovery is influenced by the mode of plasma-resin contact (batch, continuous, or packed column).