Related Experiment Video

Updated: Apr 7, 2026

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
05:50

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Published on: November 1, 2021

3.0K

Pyrin-PSTPIP1 colocalises at the leading edge during cell migration

Yeliz Z Akkaya-Ulum1, Banu Balci-Peynircioglu1, Nuhan Purali2

  • 1Department of Medical Biology, Faculty of Medicine, Hacettepe University, Sihhiye, Ankara, 06100, Turkey.

Cell Biology International
|July 17, 2015
PubMed

Insights

Familial Mediterranean Fever (FMF) is linked to MEFV gene mutations. This study reveals pyrin interacts with PSTPIP1 and actin during neutrophil migration, clarifying their roles in inflammation.

Area of Science:

  • Immunology
  • Cell Biology
  • Genetics

Background:

  • Familial Mediterranean Fever (FMF) is an auto-inflammatory disease caused by mutations in the Mediterranean Fever (MEFV) gene.
  • The MEFV gene encodes pyrin, a protein crucial for inflammatory pathways, expressed in neutrophils.
  • Pyrin's interaction with proline-serine-threonine phosphatase-interacting protein 1 (PSTPIP1) and actin is known, but its role in cell migration remains unclear.

Purpose of the Study:

  • To investigate the interaction between pyrin and PSTPIP1 during cell migration.
  • To elucidate the role of pyrin-PSTPIP1 complex in the context of cell migration dynamics.

Main Methods:

  • Development of a cell migration assay using HL-60 cells.
  • Immunofluorescence staining to analyze pyrin-PSTPIP1 interactions in control, differentiated, and stimulated HL-60 cells.

Main Results:

  • Pyrin, PSTPIP1, and polymerized actin colocalized in stimulated HL-60 cells.
  • Pyrin was observed to colocalize with actin and PSTPIP1 at the leading edge of migrating cells.
  • PSTPIP1 was found to interact with dynamic actin and pyrin at the cell's polarization site.

Conclusions:

  • This study demonstrates the colocalization of pyrin, PSTPIP1, and actin during neutrophil migration.
  • The findings provide novel insights into the interaction of pyrin and PSTPIP1 at the leading edge of migrating cells.
  • This research clarifies the molecular interactions underlying cell migration in the context of FMF and related inflammatory processes.
Keywords:
actincell migration

More Related Videos

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading10:54

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading

Published on: May 22, 2021

6.2K
Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration10:43

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

7.1K

Related Concept Videos

Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.7K
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
19.2K
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
7.5K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
4.0K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.4K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
4.1K