Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

3.3K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.3K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

4.0K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
4.0K
Master Transcription Regulators02:23

Master Transcription Regulators

8.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

4.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
4.4K
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

4.4K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
4.4K
General Transcription Factors01:30

General Transcription Factors

7.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo modeling of human γδ T cell ontogeny reveals terminal deoxynucleotidyl transferase as a key regulator of type 3 Vδ2 T cell development.

Cell reports·2026
Same author

Inhibition of A2AR alleviates adenosine-mediated suppression of plasma cell differentiation.

Frontiers in immunology·2025
Same author

IFNγ-Induced PD-L1+MHCII+ Macrophages and Tim-3+ Tumor-Reactive CD8+ T Cells Predict a Response to Anti-PD-1 Therapy in Tumor-Bearing Mice.

Cancer immunology research·2025
Same author

Generation and characterization of chicken monocyte-derived dendritic cells.

Frontiers in immunology·2025
Same author

The CD27/CD70 pathway negatively regulates visceral adipose tissue-resident Th2 cells and controls metabolic homeostasis.

Cell reports·2024
Same author

A Metabolic Gene Survey Pinpoints Fucosylation as a Key Pathway Underlying the Suppressive Function of Regulatory T Cells in Cancer.

Cancer immunology research·2023

Related Experiment Video

Updated: Apr 7, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

55.0K

Cutting Edge: Hypoxia-Inducible Factor 1 Negatively Regulates Th1 Function.

Hussein Shehade1, Valérie Acolty1, Muriel Moser1

  • 1Laboratoire d'Immunobiologie, Université Libre de Bruxelles, 6041 Gosselies, Belgium.

Journal of Immunology (Baltimore, Md. : 1950)
|July 17, 2015
PubMed
Summary
This summary is machine-generated.

Hypoxia-inducible factor (HIF)-1α regulates T cell adaptation to low oxygen. HIF-1α deficiency prevents hypoxia-induced loss of IFN-γ production in Th1 cells, revealing a key role in immune response.

More Related Videos

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation
07:46

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

4.7K
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

35.8K

Related Experiment Videos

Last Updated: Apr 7, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

55.0K
Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation
07:46

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

4.7K
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

35.8K

Area of Science:

  • Immunology
  • Cellular Biology
  • Physiology

Background:

  • Tissue hypoxia is a condition of reduced oxygen availability.
  • Hypoxia-inducible factor (HIF)-1α is a transcription factor that regulates cellular adaptation to hypoxia.
  • The role of HIF-1α in T cell fate determination is not fully understood.

Purpose of the Study:

  • To investigate whether HIF-1α regulates T cell fate.
  • To elucidate the molecular mechanisms by which HIF-1α controls T cell responses under hypoxic conditions.

Main Methods:

  • Culture of Th1 cells under normoxic and hypoxic conditions.
  • Assessment of IFN-γ production in wild-type and HIF-1α-deficient Th1 cells.
  • Measurement of IL-10 expression and STAT3 phosphorylation.
  • Analysis of suppressor of cytokine signaling 3 (SOCS3) transcription.

Main Results:

  • Hypoxia impairs the ability of Th1 cells to produce IFN-γ.
  • Th1 cells lacking HIF-1α are resistant to hypoxia-induced suppression of IFN-γ.
  • IL-10 expression is upregulated under hypoxia, and IL-10-deficient Th1 cells maintain IFN-γ production in hypoxia.
  • STAT3 phosphorylation is increased in Th1 cells under hypoxia, leading to enhanced HIF-1α transcription and potentially inhibiting SOCS3 transcription.

Conclusions:

  • HIF-1α plays a critical role in regulating Th1 cell responses to hypoxia.
  • A positive-feedback loop involving STAT3, HIF-1α, and IL-10 downregulates Th1 responses under hypoxic conditions.
  • This regulatory mechanism may prevent host tissue damage during hypoxia.