Portable Colorimetric Paper-Based Biosensing Device for the Assessment of Bisphenol A in Indoor Dust

Bisphenol A (BPA) is found in polycarbonate plastic and epoxy resins and is used in a variety of commercial and consumer products. The leaching of BPA can result in human exposure via inhalation, ingestion, and dermal routes. As a result, humans have been exposed in their home and work environment to BPA. Conventional methods for BPA exposure assessment rely on cumbersome laboratory instrumentation with high capital and operational expenditures which limit the number of samples that can be analyzed. We report here the design of a compact portable colorimetric paper-based biosensing device with integrated sampling/analysis units for field-based measurements of BPA in indoor dust. The system employs interchangeable low-cost paper-based enzyme sensors as a test zone for BPA detection interfaced with an air-sampling cassette as a sample collection area. The sensor response was concentration-dependent with a detection limit of 0.28 μg/g. The sensor was validated with the conventional gas chromatography method and used to detect BPA exposure in household dust. BPA concentrations ranged from 0.05 to 3.87 μg/g in 57 household dust samples when both methods were used. The potential of this method for field measurements of dust samples is discussed.