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[Analysis of the body fluids using SDS-gradient gel electrophoresis]
Abstract:
In the present report we describe a horizontal SDS-electrophoresis in thin- or ultrathin-layer polyacrylamide pore-gradient gel polymerized on polyester films for the estimation of proteins in several human body fluids. Up to 25 samples can be analyzed side by side under identical conditions. The combination of Coomassie blue staining with silver-staining allows the analysis of fluids containing low protein content also without concentrative techniques.