Jove
Visualize
Contact Us

Related Concept Videos

Other Unique Bacteria01:18

Other Unique Bacteria

541
Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic...
541
Homologous Recombination02:31

Homologous Recombination

65.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.7K
Homologous Recombination02:31

Homologous Recombination

7.4K
7.4K
DNA Helicases00:55

DNA Helicases

25.0K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
25.0K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

5.8K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.8K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

42.5K
Overview
42.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Switching to camizestrant at ESR1 mutation emergence before disease progression during first-line treatment of hormone receptor-positive advanced breast cancer (SERENA-6): extended analysis of a double-blind, placebo-controlled, randomised, phase 3 trial.

The Lancet. Oncology·2026
Same author

Cognitive Fluctuations, Neuropsychiatric Burden, and Quality of Life in Moderate-Advanced Dementia With Lewy Bodies: The Role of Dysautonomia.

Neurology·2026
Same author

<i>ESR1</i> ctDNA testing: literature review and comparison of assays for <i>ESR1</i> mutation detection in advanced hormone receptor-positive breast cancer.

Expert review of molecular diagnostics·2025
Same author

Multimorbidity in adults with epilepsy attending an outpatient epilepsy clinic.

Epilepsy & behavior : E&B·2025
Same author

Psychotropic Polypharmacy in Dementia: A Retrospective Analysis for People with Neuropsychiatric Symptoms Referred to an Australian Dementia Support Service.

Drugs & aging·2025
Same author

Long-term, home-based transcranial direct current stimulation coupled with computerized cognitive training in frontotemporal dementia: A case report.

Journal of central nervous system disease·2024
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 7, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

11.4K

Deinococcus radiodurans PriA is a Pseudohelicase.

Matthew E Lopper1, Jacob Boone1, Christopher Morrow1

  • 1Department of Chemistry, University of Dayton, Dayton, OH, United States of America.

Plos One
|July 17, 2015
PubMed
Summary

The bacterial PriA helicase typically unwinds DNA to restart replication forks. However, Deinococcus radiodurans PriA functions without this helicase activity, suggesting a conserved role in DNA repair.

Area of Science:

  • * Molecular biology
  • * Biochemistry
  • * Microbiology

Background:

  • * PriA helicase is crucial for restarting repaired DNA replication forks in bacteria.
  • * Its conserved helicase activity suggests a selective advantage, though not essential for viability in E. coli.
  • * Sequence motifs for helicase activity are not conserved in the Deinococcus-Thermus phylum.

Purpose of the Study:

  • * To investigate the function of PriA from Deinococcus radiodurans, a radiation-resistant bacterium.
  • * To determine if D. radiodurans PriA possesses helicase activity.
  • * To explore the interaction of D. radiodurans PriA with other replication proteins.

Main Methods:

  • * Biochemical assays to test ATP hydrolysis and DNA unwinding.
  • * Analysis of PriA sequence conservation.

More Related Videos

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

11.2K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.4K

Related Experiment Videos

Last Updated: Apr 7, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

11.4K
Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

11.2K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.4K
  • * Investigation of protein-protein interactions using co-immunoprecipitation or similar techniques.

    • Main Results:

    • * Deinococcus radiodurans PriA lacks ATP hydrolysis and DNA unwinding (helicase) activity, classifying it as a pseudohelicase.
    • * D. radiodurans PriA retains DNA binding capabilities.
    • * Evidence suggests a physical interaction between D. radiodurans PriA and the DnaB replicative helicase.

    Conclusions:

    • * PriA can maintain its role in replisome reloading at repaired replication forks even without helicase activity.
    • * This finding highlights functional divergence within conserved protein families.
    • * D. radiodurans PriA's pseudohelicase nature offers insights into DNA repair mechanisms in extreme environments.