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Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
Published on: September 20, 2014
The Drosophila histone demethylase dKDM5/LID regulates hematopoietic development
Tomás Morán1, Jordi Bernués1, Fernando Azorín1
1Institute of Molecular Biology of Barcelona, CSIC, Baldiri Reixac, 4, 08028 Barcelona, Spain; Institute for Research in Biomedicine, IRB Barcelona, Baldiri Reixac, 10, 08028 Barcelona, Spain.
Abstract:
dKDM5/LID regulates transcription of essential developmental genes and, thus, is required for different developmental processes. Here, we report the essential contribution of dKDM5/LID to hematopoiesis in Drosophila. Our results show that dKDM5/LID is abundant in hemocytes and that its depletion induces over-proliferation and differentiation defects of larval hemocytes and disrupts organization of the actin cytoskeleton. We also show that dKDM5/LID regulates expression of key factors of hematopoietic development. In particular, dKDM5/LID depletion up-regulates expression of several transcription factors involved in hemocytes proliferation and differentiation as well as of several small-GTPases that link signaling effectors to actin cytoskeleton formation and dynamics.
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