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Published on: November 23, 2012
Complete Genome Sequence of Streptococcus pyogenes emm28 Clinical Isolate M28PF1, Responsible for a Puerperal Fever
Magalie Longo, Mathieu De Jode, Céline Plainvert
1INSERM U 1016, Institut Cochin, Unité FRM Barrières et Pathogènes, Paris, France CNRS UMR 8104, Paris, France Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
Abstract:
We report the sequence of the Streptococcus pyogenes emm28 strain M28PF1, isolated from a patient with postpartum endometritis. The M28 protein is smaller than that of MGAS6180 (NC_007296.1). Furthermore, the 1,896,976-bp-long chromosome presents, compared to that of MGAS6180, an inversion between the two comX genes.
Insights
We sequenced the Streptococcus pyogenes emm28 strain M28PF1, finding its M28 protein is smaller and its chromosome has an inversion between comX genes compared to strain MGAS6180.
Area of Science:
- Microbiology
- Genomics
- Bacterial Pathogenesis
Background:
- Streptococcus pyogenes is a significant human pathogen.
- emm gene sequencing is crucial for understanding S. pyogenes epidemiology and virulence.
- Postpartum endometritis is a serious infection that can be caused by S. pyogenes.
Purpose of the Study:
- To report the complete genome sequence of the emm28 S. pyogenes strain M28PF1.
- To compare the genomic features of M28PF1 with other S. pyogenes strains, specifically MGAS6180.
- To identify genetic differences that may influence M28PF1's characteristics or virulence.
Main Methods:
- Whole-genome sequencing of Streptococcus pyogenes strain M28PF1.
- Bioinformatic analysis and comparison of the M28PF1 genome with the reference strain MGAS6180 (NC_007296.1).
- Identification and characterization of key genetic elements, including protein sequences and chromosomal structure.
Main Results:
- The genome sequence of Streptococcus pyogenes emm28 strain M28PF1 was determined.
- The M28 protein in strain M28PF1 was found to be smaller than that of strain MGAS6180.
- A chromosomal inversion between the two comX genes was identified in M28PF1 when compared to MGAS6180.
Conclusions:
- The M28PF1 strain represents a distinct genetic variant of emm28 Streptococcus pyogenes.
- The observed differences in M28 protein size and chromosomal structure may have implications for strain behavior and pathogenesis.
- Further research is warranted to explore the functional consequences of these genomic variations.
