[In vitro growth of eosinophil colonies from normal human peripheral blood]

Eosinophil colony formation was observed in culture of normal peripheral mononuclear cells in the presence of phytohemagglutinin stimulated peripheral mononuclear cell conditioned medium. The number of eosinophil colonies from 10(6) mononuclear cells varied ranging between 2 and 95, and the mean number was 20.4 +/- 6.3 (SE). A large proportion of total colonies was eosinophil colonies accounted for 81.2 +/- 10.7% of the total colonies.