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Updated: Apr 6, 2026

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Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
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Identification of ribonucleotide reductase mutation causing temperature-sensitivity of herpes simplex virus isolates
Tohru Daikoku1, Yukari Oyama1, Misako Yajima1
1Department of Virology, University of Toyama Toyama, 930-0194, Japan.
Clinical Case Reports
|July 18, 2015
Abstract:
Herpes simplex virus 2 caused a genital ulcer, and a secondary herpetic whitlow appeared during acyclovir therapy. The secondary and recurrent whitlow isolates were acyclovir-resistant and temperature-sensitive in contrast to a genital isolate. We identified the ribonucleotide reductase mutation responsible for temperature-sensitivity by deep-sequencing analysis.
Keywords:
Herpes simplex virushigh-throughput DNA sequencingreactivationribonucleotide reductasetemperature-sensitive (Ts)thymidine kinase deficient
