Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

61.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
61.7K
RNA Splicing01:32

RNA Splicing

20.3K
20.3K
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

7.4K
7.4K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.7K
3.7K
Alternative RNA Splicing02:18

Alternative RNA Splicing

27.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
27.0K
Alternative RNA Splicing02:18

Alternative RNA Splicing

5.5K
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural basis of the regulation by CDK11 kinase of early spliceosome activation and evidence for its proofreading by DHX15 helicase.

Nature communications·2026
Same author

The co-localizing Zorya II, Druantia III, and ARMADA II defense systems on O-island 172 confer synergistic anti-phage defense in enterohemorrhagic <i>Escherichia coli</i>.

mBio·2026
Same author

Ski2-like helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses.

Cell reports·2026
Same author

The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses.

bioRxiv : the preprint server for biology·2025
Same author

Functional investigation of the RNA helicase MOV10 with respect to its interplay with factors involved in nonsense-mediated mRNA decay.

The Journal of biological chemistry·2025
Same author

The macromolecular crystallography beamlines of the Helmholtz-Zentrum Berlin at the BESSY II storage ring: history, current status and future directions.

Journal of synchrotron radiation·2025

Related Experiment Video

Updated: Apr 6, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

3.1K

SnapShot: Spliceosome Dynamics II.

Markus C Wahl1, Reinhard Lührmann2

  • 1Laboratory of Structural Biochemistry, Freie Universität Berlin, Takustraße 6, 14195 Berlin, Germany.

Cell
|July 18, 2015
PubMed
Summary
This summary is machine-generated.

Spliceosomes regulate gene expression through dynamic mechanisms, enabling alternative splicing for complex gene repertoires. Understanding these processes is crucial for deciphering cellular complexity and disease.

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.4K
Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
05:44

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Published on: November 9, 2020

4.2K

Related Experiment Videos

Last Updated: Apr 6, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

3.1K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.4K
Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
05:44

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Published on: November 9, 2020

4.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Alternative splicing is a key mechanism in higher eukaryotes, allowing a single gene to encode multiple proteins.
  • Spliceosomes, the molecular machines responsible for splicing, exhibit complex conformational and compositional dynamics.
  • Accurate splice site recognition is essential, yet flexibility is required for alternative splicing.

Purpose of the Study:

  • To explore the mechanisms regulating spliceosome dynamics.
  • To understand how splice site selection is controlled during alternative splicing.
  • To investigate the existence and role of minor spliceosomes.

Main Methods:

  • Analysis of spliceosome conformational changes.
  • Investigating pre-mRNA-spliceosome interactions.
  • Comparative genomics to study spliceosome diversity.

Main Results:

  • Identified multiple mechanisms modulating spliceosome dynamics for splicing regulation.
  • Demonstrated principles ensuring both accurate and flexible splice site identification.
  • Confirmed the presence of a U12-type minor spliceosome in some species.

Conclusions:

  • Spliceosome dynamics are central to regulating gene expression via alternative splicing.
  • A balance of fidelity and flexibility in splice site recognition is achieved through diverse mechanisms.
  • The existence of distinct spliceosome types highlights the complexity of RNA processing.