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Advanced Caries Microbiota in Teeth with Irreversible Pulpitis.

Isabela N Rôças¹, Kenio C Lima², Isauremi V Assunção²

¹Department of Endodontics and Molecular Microbiology Laboratory, Estácio de Sá University, Rio de Janeiro, Rio de Janeiro, Brazil.

Journal of Endodontics

|July 19, 2015

Summary

Specific bacteria like Atopobium genomospecies C1 and Streptococcus mutans were identified in deep tooth decay linked to irreversible pulpitis. Certain bacterial levels correlated with specific pain symptoms, suggesting their role in endodontic infection.

Keywords:

Dentinal caries Lactobacillus Streptococcus irreversible pulpitis microbiota molecular biology permanent teeth

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Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

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Published on: May 17, 2024

Area of Science:

Endodontics
Microbiology
Cariology

Background:

Bacterial invasion of the pulp initiates endodontic infection.
The microbiota of deep dentinal caries in irreversible pulpitis requires further investigation.

Purpose of the Study:

To identify bacterial taxa in advanced dentinal caries lesions associated with irreversible pulpitis.
To correlate the presence and levels of specific bacteria with clinical symptoms.

Main Methods:

DNA analysis of deep caries samples using reverse-capture checkerboard hybridization.
Quantification of total bacteria, streptococci, and lactobacilli via real-time quantitative polymerase chain reaction.
Evaluation of associations between bacterial taxa and clinical signs/symptoms.

Main Results:

Atopobium genomospecies C1, Pseudoramibacter alactolyticus, and Streptococcus species were frequently detected.
Streptococcus species, Dialister invisus, and Parvimonas micra correlated with throbbing pain.
Streptococcus mutans and Lactobacillus species were associated with pain on percussion and continuous pain, respectively.
Mean total bacterial load was 1 x 10^8 cell equivalents/mg dentin.

Conclusions:

Advanced caries lesions harbor specific bacterial taxa.
Certain bacteria are significantly associated with irreversible pulpitis symptoms.
These findings suggest a role for these taxa in the etiology of irreversible pulpitis.