Related Experiment Video
Updated: Apr 6, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Structural modeling for DNA binding to antioxidants resveratrol, genistein and curcumin
C N N'soukpoé-Kossi1, P Bourassa1, J S Mandeville1
1Department of Chemistry-Physics, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières, Québec G9A 5H7, Canada.
Abstract:
Several models are presented here for the bindings of the antioxidant polyphenols resveratrol, genistein and curcumin with DNA in aqueous solution at physiological conditions. Multiple spectroscopic methods and molecular modeling were used to locate the binding sites of these polyphenols with DNA duplex. Structural models showed that intercalation is more stable for resveratrol and genistein than groove bindings, while curcumin interaction is via DNA grooves. Docking showed more stable complexes formed with resveratrol and genistein than curcumin with the free binding energies of -4.62 for resveratrol-DNA (intercalation), -4.28 for resveratrol-DNA (groove binding), -4.54 for genistein-DNA (intercalation), -4.38 for genistein-DNA (groove binding) and -3.84 kcal/mol for curcumin-DNA (groove binding). The free binding energies show polyphenol-DNA complexation is spontaneous at room temperature. At high polyphenol concentration a major DNA aggregation occurred, while biopolymer remained in B-family structure.
Related Concept Videos
Cooperative Binding of Transcription Regulators
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
Conserved Binding Sites
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Overview of DNA Repair
Chemically...
DNA Damage can Stall the Cell Cycle

