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Related Experiment Video

Updated: Apr 6, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
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Engineering an analog-sensitive CDK12 cell line using CRISPR/Cas.

Bartlomiej Bartkowiak1, Christopher Yan1, Arno L Greenleaf1

  • 1Department of Biochemistry, Duke University Medical Center, United States.

Biochimica Et Biophysica Acta
|July 21, 2015
PubMed
Summary
This summary is machine-generated.

Researchers engineered an analog-sensitive CDK12 cell line to study its function. This tool helps understand CDK12

Keywords:
Analog-sensitiveCDK12CRISPR/CasCTD phosphorylationCell lineUnintended splice site

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • CDK12, a kinase regulating RNA Polymerase II, is linked to tumor suppression and DNA damage response.
  • Understanding CDK12 function is hindered by the lack of specific inhibitors and challenges with RNAi.
  • New tools are needed to investigate CDK12's precise molecular roles.

Purpose of the Study:

  • To develop a novel tool for studying CDK12 function.
  • To enable selective and reversible inhibition of CDK12 activity.

Main Methods:

  • Utilized CRISPR/Cas gene editing to create an analog-sensitive CDK12 (asCDK12) mammalian cell line.
  • Developed a system for inducible inhibition of CDK12 using a cell-permeable adenine analog.

Main Results:

  • Inhibition of asCDK12 led to altered CTD phosphorylation patterns.
  • CDK12 inhibition resulted in a significant arrest of cellular proliferation.

Conclusions:

  • The developed asCDK12 cell line is a powerful new tool for dissecting CDK12 function.
  • This system facilitates future research into CDK12's roles in cellular processes and disease.