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Updated: Apr 6, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes
Published on: May 4, 2020
Hydrogen deuterium mass spectrometry in drug discovery
Nino Campobasso1, Donald Huddler1
1GlaxoSmithKline R&D, Molecular Discovery Research, US-Biophysics, Collegeville, PA 19426, USA.
Abstract:
Hydrogen deuterium exchange mass spectrometry (HDX-MS) has become an increasingly routine technology for probing the solution structure and dynamics of proteins. HDX-MS measures the exchange of deuterium with the amide proton on the protein backbone chain. HDX-MS results can be used to aid in construct design for biophysical studies, to probe protein-ligand interactions and to characterize the dynamics of proteins. This digest highlights recent progress which makes this technology accessible and explores examples of HDX-MS in drug discovery and solution state structural biology.
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