Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

4.2K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.2K
Epigenetic Regulation01:46

Epigenetic Regulation

34.3K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.3K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

9.1K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
9.1K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

10.1K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
10.1K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

3.0K
3.0K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

11.4K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome reorganization and its functional impact during breast cancer progression.

eLife·2026
Same author

PABPC1 Modulates Immunoglobulin pre-mRNA Alternative Polyadenylation.

bioRxiv : the preprint server for biology·2026
Same author

Intranasal HSV-1 Infection Drives Region-Specific Interferon-Dominant Microglial Remodeling.

bioRxiv : the preprint server for biology·2026
Same author

Acute degron-mediated RUNX1 loss reprograms enhancer activity to epigenetically drive epithelial destabilization and initiate cancer hallmarks.

bioRxiv : the preprint server for biology·2026
Same author

IKAROS Gene Regulatory Network Reveal ERG as a Vulnerability in B-cell Acute Lymphoblastic Leukemia.

bioRxiv : the preprint server for biology·2026
Same author

Correction: Mejía-García et al. Development and Validation of an Extracellular Matrix Gene Expression Signature for Prognostic Prediction in Patients with Uveal Melanoma. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 4317.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Apr 6, 2026

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

11.0K

Altering cancer transcriptomes using epigenomic inhibitors.

Malaina Gaddis1, Diana Gerrard2, Seth Frietze2

  • 1USC/Norris Comprehensive Cancer Center, University of Southern California, 1450 Biggy Street, NRT 6503, Los Angeles, CA 90089-9601 USA.

Epigenetics & Chromatin
|July 21, 2015
PubMed
Summary
This summary is machine-generated.

Epigenetic inhibitors, including CBP-specific drug ICG-001, impact WNT signaling and gene expression in colon cancer cells. General HAT inhibition with C646 mirrors CBP inhibition, affecting WNT pathways and reversing tumor-specific gene expression changes.

Keywords:
C646Cholesterol biosynthesisColon cancerEpigenetic inhibitorHistone acetylationICG-001Pancreatic cancerTCF7L2WNT signaling

More Related Videos

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.0K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.2K

Related Experiment Videos

Last Updated: Apr 6, 2026

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

11.0K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.0K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.2K

Area of Science:

  • Oncology
  • Epigenetics
  • Molecular Biology

Background:

  • WNT signaling hyper-activation is common in various cancers, driving the development of pathway-specific inhibitors.
  • Epigenetic inhibitors show promise for WNT pathway inhibition.
  • The WNT pathway involves beta-catenin (CTNNB1), TCF transcription factors, and co-activators CBP (CREBBP) and p300 (EP300), regulating gene expression tied to proliferation and differentiation.

Purpose of the Study:

  • To compare the genome-wide transcriptomic effects of a CBP-specific inhibitor (ICG-001) with a general CBP/p300 inhibitor (C646).
  • To investigate the impact of these epigenetic inhibitors on WNT signaling and other cellular pathways in colon and pancreatic cancer cells.

Main Methods:

  • Genome-wide transcriptome analysis was performed on HCT116 colon cancer cells and PANC1 pancreatic cancer cells.
  • Cells were treated with ICG-001 (CBP-specific inhibitor) and C646 (general HAT inhibitor).

Main Results:

  • Both ICG-001 and C646 induced significant changes in the transcriptome of both cancer cell lines, reversing some tumor-specific gene expression.
  • While both drugs affected cell cycle pathways, WNT signaling was only impacted in colon cancer cells (HCT116).
  • Treatment of HCT116 cells with C646 resulted in downregulation of WNT target genes, similar to the effect of ICG-001.

Conclusions:

  • General HAT inhibition produces transcriptomic effects comparable to CBP-specific inhibition.
  • Epigenetic inhibition impacts the WNT pathway in HCT116 colon cancer cells and the cholesterol biosynthesis pathway in PANC1 pancreatic cancer cells.