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Guiqi polysaccharide protects the normal human fetal lung fibroblast WI-38 cells from H2O2-induced premature
Xiuying Pu1, Shuang Yu1, Wenbo Fan1
1College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, Gansu, China; The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, Gansu Educational Department Lanzhou 730050, China.
Objective:
This study is to investigate the effects of Guiqi polysaccharide (GQP) on H2O2-induced premature senescence in normal human fetal lung fibroblast WI-38 cells.
Methods:
WI-38 cells were subjected to treatments of GQP, Angelica sinensis polysaccharide (ASP), and Astragalus membranaceus polysaccharide (AMP), and then treated with H2O2 to induce premature senescence. Morphological observation, MTT assay, senescence-associated β-galactosidase activity assessment, telomerase activity determination, cell cycle analysis, and Western blot analysis were performed to evaluate cellular senescence.
Results:
H2O2 treatment induced premature senescence in WI-38 cells, as indicated by the decreased fibroblast proliferation activity and changed cellular morphology. When treated with GQP, ASP, or AMP, the morphological changes in WI-38 cells induced by H2O2 could be restored. SA-β-gal activity was elevated in H2O2-treated WI-38 cells, which could be decreased by GQP treatment. Moreover, compared with the normal control, H2O2 treatment significantly inhibited the telomerase activity of WI-38 cells. However, GQP effectively elevated the telomerase activity of these senescent cells. Furthermore, flow cytometry and cell cycle analysis showed that GQP treatment could abrogate the cell cycle arrest in H2O2-treated WI-38 cells, which might contribute to the anti-senescent effects. In addition, GQP significantly affected the p53-p21 and p16-pRb pathways in H2O2-treated WI-38 cells. The effectiveness of GQP was superior to AMP or ASP treatment alone.
Conclusion:
GQP has protective effects in oxidative stress-induced senescence. Our findings suggest the promising role of GQP as an attractive and bio-safe agent with the potential to retard senescence and attenuate senescence-related diseases.
