Related Experiment Video

Updated: Apr 6, 2026

Author Spotlight: Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue
09:18

Author Spotlight: Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

Published on: February 24, 2023

10.9K

Expression and function of microRNA-188-5p in activated rheumatoid arthritis synovial fibroblasts

Anke Ruedel1, Peter Dietrich2, Thomas Schubert3

  • 1Institute of Pathology, University Hospital Regensburg Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany.

International Journal of Clinical and Experimental Pathology
|July 21, 2015
PubMed

Insights

MicroRNA-188-5p is downregulated in rheumatoid arthritis (RA) synovial fibroblasts, inhibiting their migration and regulating extracellular matrix genes. This suggests miR-188-5p as a potential therapeutic target for RA treatment.

Area of Science:

  • Immunology
  • Molecular Biology
  • Rheumatology

Background:

  • Activated synovial fibroblasts (RASF) are key drivers of rheumatoid arthritis (RA) pathology.
  • MicroRNA (miR) deregulation is implicated in RA development and progression.

Purpose of the Study:

  • Identify dysregulated miRs in RASF.
  • Identify target genes contributing to the aggressive RASF phenotype.
  • Investigate the role of miR-188-5p in RA pathogenesis.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure miR-188-5p expression.
  • Transient transfection to re-express miR-188-5p in RASF.
  • Gene expression profiling and in silico analysis to identify miR-188-5p targets.

Main Results:

  • miR-188-5p was significantly downregulated in RA synovial tissues and RASF.
  • Interleukin-1β further reduced miR-188-5p expression.
  • Re-expression of miR-188-5p inhibited RASF migration but not GAG degradation or repellent factor expression.
  • miR-188-5p directly targets KIAA1199 and indirectly regulates COL1A1 and COL12A1.

Conclusions:

  • miR-188-5p is downregulated in RA, potentially due to inflammation, and inhibits RASF migration.
  • miR-188-5p regulates genes involved in extracellular matrix remodeling in RA.
  • miR-188-5p represents a potential therapeutic target for inhibiting RA progression.
Keywords:
KIAA1199Rheumatoid arthritismiR-188-5psynovial fibroblasts

More Related Videos

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice07:37

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

1.4K
Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients09:31

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients

Published on: October 16, 2016

10.0K

Related Concept Videos

The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
14.0K
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.7K
MicroRNAs01:22

MicroRNAs

12.1K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
11.0K