Related Experiment Video
Updated: Apr 6, 2026
09:17
Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy
Published on: September 16, 2019
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Inhibitory effect of midkine-binding peptide on tumor proliferation and migration
Hui-Lian Huang1, Jian-Fen Shen2, Li-Shan Min1
1Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou 313000, China.
Summary
Midkine-binding peptides effectively inhibit human umbilical vein endothelial cell proliferation and suppress tumor angiogenesis in a xenograft model. These peptides show potential as anti-angiogenesis agents for solid tumor treatment.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Midkine plays a crucial role in tumor angiogenesis and cell proliferation.
- Targeting midkine is a potential strategy for cancer therapy.
Purpose of the Study:
- To investigate the anti-angiogenic and anti-proliferative effects of midkine-binding peptides.
- To evaluate the efficacy of midkine-binding peptides in a xenograft tumor model.
Main Methods:
- Phage display was used to identify midkine-binding peptides.
- Peptide binding activity was confirmed using ELISA.
- In vitro proliferation assays (MTT) and in vivo xenograft models (H22 hepatocarcinoma in mice) were employed.
- Immunohistochemistry for factor VIII staining assessed microvessel density.
Main Results:
- Specific midkine-binding peptides significantly inhibited human umbilical vein endothelial cell (HUVEC) proliferation.
- These peptides demonstrated potent suppression of angiogenesis induced by H22 hepatocarcinoma cells in mice.
- Microvessel density in tumors was reduced by the midkine-binding peptides.
Conclusions:
- Midkine-binding peptides effectively inhibit tumor growth by impeding new blood vessel formation.
- These peptides hold promise as novel anti-angiogenesis therapeutics for solid tumors.
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