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Related Concept Videos

Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

7.4K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
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IR Spectrometers01:25

IR Spectrometers

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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

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IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
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IR Spectrum01:19

IR Spectrum

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When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...
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Related Experiment Video

Updated: Apr 6, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
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A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

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Wide-field FTIR microscopy using mid-IR pulse shaping.

Arnaldo L Serrano, Ayanjeet Ghosh, Joshua S Ostrander

    Optics Express
    |July 21, 2015
    PubMed
    Summary
    This summary is machine-generated.

    A new table-top Fourier transform infrared (FTIR) microscopy technique uses a femtosecond pulse shaper and focal plane array to rapidly collect high-quality, sub-diffraction limited chemical images, resolving distinct species.

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    Area of Science:

    • Spectroscopy
    • Microscopy
    • Chemical Imaging

    Background:

    • Fourier transform infrared (FTIR) microscopy is crucial for chemical analysis.
    • Conventional FTIR microscopes can be limited by speed and resolution.

    Purpose of the Study:

    • To develop a novel, rapid, table-top FTIR microscopy technique.
    • To achieve sub-diffraction limited chemical imaging with high signal-to-noise.

    Main Methods:

    • Combining a femtosecond pulse shaper with a mid-infrared focal plane array.
    • Utilizing rapid delay scanning and phase control for interferogram acquisition.
    • Employing refractive objectives for optimal coherent radiation imaging.

    Main Results:

    • Successfully collected wide-field FTIR microscopic images of polystyrene beads with W(CO)₆ or Mn₂(CO)₁₀.
    • Demonstrated spatial resolution of chemically distinct species.
    • Achieved sub-diffraction limited imaging, verified by USAF test targets and simulations.

    Conclusions:

    • The developed table-top FTIR microscopy technique offers a rapid and high-resolution alternative.
    • This method enables precise spatial resolution of chemical compositions.
    • Refractive objectives are recommended for imaging with coherent infrared radiation.