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Updated: Apr 6, 2026

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
Published on: June 2, 2022
Engineered CHO cells for production of diverse, homogeneous glycoproteins
Zhang Yang1, Shengjun Wang1, Adnan Halim1
11] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] The Novo Nordisk Foundation Center for Biosustainability, The Danish Technical University, Denmark.
Abstract:
Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.
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