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Site-directed spin labeling of 2'-amino groups in RNA with isoindoline nitroxides that are resistant to reduction
Subham Saha1, Anil P Jagtap, Snorri Th Sigurdsson
1University of Iceland, Department of Chemistry, Science Institute, Dunhaga 3, 107 Reykjavik, Iceland. snorrisi@hi.is.
Chemical Communications (Cambridge, England)
|July 21, 2015
Abstract:
Two aromatic isothiocyanates, derived from isoindoline nitroxides, were synthesized and selectively reacted with 2'-amino groups in RNA. The spin labels displayed limited mobility in RNA, making them promising candidates for distance measurements by pulsed EPR. After conjugation to RNA, a tetraethyl isoindoline derivative showed significant stability under reducing conditions.
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