Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Fibroblasts01:09

Introduction to Fibroblasts

4.4K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
4.4K
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

7.2K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
7.2K
The Role of Actin and Myosin in Non-muscle Cells01:10

The Role of Actin and Myosin in Non-muscle Cells

5.8K
Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
5.8K
Microscopic Anatomy of Skeletal Muscles01:13

Microscopic Anatomy of Skeletal Muscles

29.7K
Skeletal muscle cells, also called muscle fibers, are distinctly elongated, multi-nucleated, slender biological units. They are packed with specialized structures designed to facilitate their primary function, which is contraction.
The muscle sarcolemma is a plasma membrane enclosing each muscle cell that conducts electrical signals called action potentials. The sarcolemma extends into the cell to form T-tubules, ensuring the neural impulses are uniformly distributed across the entire muscle...
29.7K
Connective Tissue Cell Types01:22

Connective Tissue Cell Types

4.5K
Connective tissue develops from the mesoderm of a developing embryo and consists of cells, fibers, and ground substance: a gel-like material containing large complexes of carbohydrates and proteins. Connective tissue was first identified as a separate tissue family in the 18th century, and Johannes Peter Muller coined the term connective tissue.
Fat cells (adipocytes), smooth muscle cells (myoblasts), and bone cells (osteoblasts) are some connective tissue cell types. Some immune system cells...
4.5K
Skeletal Muscle Anatomy00:55

Skeletal Muscle Anatomy

95.8K
Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.
95.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting Cellular Senescence Enhances Post-Burn Wound Healing in Aged Mice.

Shock (Augusta, Ga.)·2026
Same author

A Circuit of Mechanically Regulated Transcription Factors Balances Regenerative and Fibrotic Memory of Mesenchymal Stromal Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Identification of Ephrin type-B receptor 4 as a critical mediator of tissue fibrosis.

JCI insight·2025
Same author

Targeting myeloid cell-mediated fibrosis through FAK.

Nature biomedical engineering·2025
Same author

Microfluidic Production of Ultrathin, Handleable Collagen Sheets Exhibiting Toe-heel Tensile Behavior.

Advanced materials technologies·2025
Same author

Depletion of endomembrane reservoirs drives phagocytic appetite exhaustion in macrophages.

Journal of cell science·2025

Related Experiment Video

Updated: Apr 6, 2026

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
06:59

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Published on: October 12, 2013

20.8K

Myofibroblasts.

Boris Hinz1

  • 1Laboratory of Tissue Repair and Regeneration, Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, 150 College Street, FitzGerald Building, Room 234, Toronto, M5S 3E2 Ontario, Canada.

Experimental Eye Research
|July 21, 2015
PubMed
Summary
This summary is machine-generated.

Myofibroblasts are key to wound repair but can cause organ dysfunction through fibrosis. Understanding ocular myofibroblast behavior is crucial for treating eye conditions.

Keywords:
ContractionContractureFibrosisInflammationScarStress fiberTransforming growth factor betaα-smooth muscle actin

More Related Videos

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
10:45

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Published on: March 12, 2020

17.4K
Ultrasonic-augmented Primary Adult Fibroblast Isolation
06:51

Ultrasonic-augmented Primary Adult Fibroblast Isolation

Published on: July 29, 2019

8.4K

Related Experiment Videos

Last Updated: Apr 6, 2026

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
06:59

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Published on: October 12, 2013

20.8K
Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
10:45

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Published on: March 12, 2020

17.4K
Ultrasonic-augmented Primary Adult Fibroblast Isolation
06:51

Ultrasonic-augmented Primary Adult Fibroblast Isolation

Published on: July 29, 2019

8.4K

Area of Science:

  • Cell Biology
  • Tissue Repair
  • Ophthalmology

Background:

  • Myofibroblasts are crucial for wound healing by repairing extracellular matrix.
  • However, their persistent activation leads to fibrosis and reduced organ function.
  • Dysregulated myofibroblast activity, driven by chronic inflammation or stress, causes detrimental scarring.

Purpose of the Study:

  • To review the current literature on myofibroblasts.
  • To emphasize their role in ocular repair and fibrosis.
  • To explore factors influencing myofibroblast activation and potential control strategies.

Main Methods:

  • Literature review of myofibroblast research.
  • Focus on studies related to ocular repair and fibrosis.
  • Analysis of myofibroblast origins, activation factors, and molecular mechanisms.

Main Results:

  • Myofibroblasts contribute to scarring and fibrosis in various organs.
  • Ocular myofibroblasts share similar behaviors with those in other tissues.
  • Persistent myofibroblast activation is linked to impaired organ performance.

Conclusions:

  • Myofibroblasts play a dual role in tissue repair and fibrosis.
  • Understanding ocular myofibroblast behavior is vital for treating eye diseases.
  • Targeting myofibroblast activity may offer therapeutic potential for fibrotic conditions.