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Updated: Apr 6, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM
Published on: August 5, 2009
Speckle fields generated with binary diffusers and synthetic pupils implemented on a spatial light modulator
Abstract:
Digitally controllable Gaussian speckle fields were experimentally generated by implementing binary diffusers and synthetic pupils on a liquid crystal spatial light modulator. The synthetic pupil comprises a Ronchi phase mask and a proper filtering of its diffraction orders. The binary diffuser is displayed inside an aperture defined onto the Ronchi phase mask. We demonstrated that this implementation replaces the need of using a ground glass and a physical pupil. In this way, the average speckle size, the statistical independence among the generated speckle patterns, and the average intensity distribution can be dynamically controlled.
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