Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

6.3K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
6.3K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.6K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.6K
Stem Cell Culture01:17

Stem Cell Culture

6.6K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short histological kaleidoscope - recent findings in histology. Part VI. Kupffer cells, hepatic stellate cells, enteroendocrine cells, and telocytes.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie·2026
Same author

Comparative Effects of <i>Escherichia coli</i> vs. <i>Porphyromonas gingivalis</i> Lipopolysaccharides on Osteogenic Differentiation and the Expression of lncRNAs in Periodontal Ligament Stem Cells.

International journal of molecular sciences·2026
Same author

Circulating ACE2 levels and ACE I/D polymorphism in severe aortic stenosis.

Scientific reports·2026
Same author

Pharmacological Properties and the Impact of Caffeic Acid-Entrapped Liposomes on Triple-Negative Breast Cancer Cell Lines Exposed to Doxorubicin.

Antioxidants (Basel, Switzerland)·2026
Same author

DNA-hybridization on gold nanospheres: a dual-fluorescence investigation of surface loading and strand length effects.

Journal of biomedical optics·2026
Same author

Tunable gelatin-coated biosynthesized gold nanotriangles: Thermoplasmonic performance and in vitro therapeutic potential via on-demand visible-NIR LED activation.

Colloids and surfaces. B, Biointerfaces·2026

Related Experiment Video

Updated: Apr 6, 2026

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
08:32

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Published on: March 27, 2019

6.5K

Human placenta - stem cell source for obtaining pancreatic progenitors.

Sergiu Şuşman1, Dan Rus-Ciucă, Olga Soriţău

  • 1Department of Histology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania; serman_s@yahoo.com.

Romanian Journal of Morphology and Embryology = Revue Roumaine De Morphologie Et Embryologie
|July 21, 2015
PubMed
Summary

Placental stem cells can be transformed into pancreatic progenitors using specific differentiation protocols. This offers a potential new avenue for cell therapy in treating diabetes.

More Related Videos

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
10:12

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Published on: December 16, 2021

3.3K
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
09:23

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Published on: March 7, 2017

8.5K

Related Experiment Videos

Last Updated: Apr 6, 2026

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
08:32

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Published on: March 27, 2019

6.5K
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
10:12

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Published on: December 16, 2021

3.3K
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
09:23

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Published on: March 7, 2017

8.5K

Area of Science:

  • Stem cell biology
  • Endocrinology
  • Regenerative medicine

Background:

  • Type 1 diabetes results from insufficient insulin-producing beta cells.
  • Cell therapy using stem cells is a promising approach for diabetes treatment.
  • Understanding pancreatic development is crucial for generating functional beta cells.

Purpose of the Study:

  • To investigate the potential of amniotic membrane-derived stem cells for pancreatic progenitor generation.
  • To establish a differentiation protocol for obtaining pancreatic progenitors from placental stem cells.
  • To evaluate the immunological properties of these cells for potential transplantation.

Main Methods:

  • A three-stage differentiation protocol was applied to isolated amniotic membrane stem cells.
  • Differentiation was guided by modulating glucose concentration, substrate type (collagen + laminin), nicotinamide, and exendin-4.
  • Cellular analysis included immunofluorescence, gene expression (RT-PCR), and morphological assessment.

Main Results:

  • Differentiated cells expressed key pancreatic progenitor transcription factors (Pdx1 and PAX4).
  • Cells formed islet-like clusters and showed positive dithizone staining.
  • Immunocytochemistry confirmed insulin expression in the differentiated cells.

Conclusions:

  • Placental stem cells can be successfully differentiated into pancreatic progenitors.
  • The established protocol provides a method for generating pancreatic progenitors from amniotic membrane-derived stem cells.
  • These findings support the potential of placental stem cells in cell-based therapies for diabetes.