Determination of Human Hemoglobin Derivatives

Area of Science:

• Clinical Chemistry
• Spectrophotometry
• Hematology

Background:

• Hemoglobin (Hb) exists in active (oxyhemoglobin) and inactive pigment forms.
• Measuring these Hb forms and total concentration is crucial for assessing oxygen transport and health.
• Existing methods may lack sensitivity or accuracy for simultaneous quantification.

Purpose of the Study:

• To develop and validate a novel multi-component spectrophotometric method.
• To accurately quantify inactive Hb pigments (methemoglobin, carboxyhemoglobin, sulfohemoglobin) and oxyhemoglobin.
• To determine blood Hb concentration in healthy non-pregnant females.

Main Methods:

• Development of a multi-component spectrophotometric assay.
• Analysis of blood samples from healthy non-pregnant female volunteers.
• Quantification of oxyhemoglobin (oxyHb), methemoglobin (metHb), carboxyhemoglobin (HbCO), and sulfohemoglobin (SHb).

Main Results:

• Established reference ranges for Hb pigments: SHb% (0.0727-0.370%), metHb% (0.43-1.0%), HbCO% (0.4-1.52%), oxyHb% (97.06-98.62%).
• Determined blood Hb concentration range: 12.608-15.777 g/dL.
• The method demonstrated high sensitivity, accuracy, and reproducibility.

Conclusions:

• The new spectrophotometric method reliably quantifies multiple hemoglobin forms and concentration.
• Provides essential reference values for hemoglobin profiles in healthy women.
• Offers a sensitive and accurate tool for clinical and research applications in hematology.