Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

91.2K
Overview
91.2K
The Extracellular Matrix01:29

The Extracellular Matrix

13.3K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
13.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interfacial assembly of collagen and poly(acrylic acid) forms a dense and low-permeable collagen membrane.

Biochemical and biophysical research communications·2026
Same author

Localized stiffness programming enables tunable spatial control of vascular density in 3D hydrogels.

Chemical communications (Cambridge, England)·2026
Same author

Bubble Formation Control: Fabrication of Centimeter-Sized Tissue-Like Constructs by Catalase-Coated Oxygen-Releasing Hydrogel.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Organic acid optimization for the fabrication of centimeter-scale uniaxially aligned capillary networks in cell-laden collagen fiber assemblies.

Biochemical and biophysical research communications·2026
Same author

Phototunable hydrogel mechanics for spatial guidance of blood capillary morphogenesis.

Biofabrication·2026
Same author

Enhancement of Cell Adhesion on 3D-Printed PLA through Surface Modification Using Photoactivated Chlorine Dioxide.

ACS applied bio materials·2026

Related Experiment Video

Updated: Apr 6, 2026

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
16:33

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

Published on: April 17, 2014

13.0K

Nanometer-sized extracellular matrix coating on polymer-based scaffold for tissue engineering applications.

Noriyuki Uchida1,2, Srikanth Sivaraman3, Nicholas J Amoroso4

  • 1Department of Chemistry and Biotechnology, School of Engineering, the University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo, 113-8656, Japan.

Journal of Biomedical Materials Research. Part A
|July 22, 2015
PubMed
Summary

Surface modification using fibronectin and gelatin (FN-G) nano-layers on poly(carbonate urethane)urea (PCUU) scaffolds significantly enhanced bladder smooth muscle cell adhesion and proliferation. This technique shows promise for engineering bladder tissues in vitro.

Keywords:
artificial tissueextracellular matrixlayer-by-layerscaffoldsmooth muscle cell

More Related Videos

A Facile and Eco-friendly Route to Fabricate PolyLactic Acid Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate PolyLactic Acid Scaffolds with Graded Pore Size

Published on: October 17, 2016

9.2K
Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
11:13

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

8.8K

Related Experiment Videos

Last Updated: Apr 6, 2026

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
16:33

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

Published on: April 17, 2014

13.0K
A Facile and Eco-friendly Route to Fabricate PolyLactic Acid Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate PolyLactic Acid Scaffolds with Graded Pore Size

Published on: October 17, 2016

9.2K
Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
11:13

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

8.8K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Surface Chemistry

Background:

  • Synthetic polymer scaffolds are crucial for tissue engineering but often lack sufficient cell adhesion properties.
  • Enhancing cell interaction with scaffolds is key to successful tissue regeneration.
  • Surface modification techniques are vital for improving biocompatibility and cellular response.

Purpose of the Study:

  • To develop a novel layer-by-layer (LbL) fabrication method for creating fibronectin and gelatin (FN-G) nano-layers on poly(carbonate urethane)urea (PCUU) scaffolds.
  • To evaluate the impact of these FN-G nano-layers on the adhesion, proliferation, and behavior of bladder cells.
  • To assess the potential of this modified scaffold for bladder tissue engineering applications.

Main Methods:

  • Fabrication of PCUU scaffolds using electrospinning.
  • Layer-by-layer (LbL) deposition of fibronectin and gelatin onto PCUU scaffolds to create PCUU(FN-G) structures.
  • Scanning electron microscopy (SEM) to analyze scaffold morphology.
  • In vitro cell culture studies using bladder smooth muscle cells (BSMCs) and UROtsa cells.

Main Results:

  • LbL fabrication successfully created controlled thickness FN-G nano-layers on PCUU scaffolds without altering their 3D structure.
  • PCUU(FN-G) scaffolds demonstrated significantly improved adhesion and proliferation of BSMCs compared to unmodified PCUU scaffolds.
  • UROtsa cells cultured on PCUU(FN-G) formed a thick, multilayered structure with cell-to-cell contacts, unlike on PCUU scaffolds where many cells died.
  • Adherent BSMCs exhibited migration onto the PCUU(FN-G) scaffold, indicating high cell affinity.

Conclusions:

  • The LbL deposition of fibronectin and gelatin nano-layers is an effective surface modification strategy for enhancing cell adhesion and proliferation on PCUU scaffolds.
  • This approach shows significant potential for advancing the in vitro engineering of bladder tissues.
  • The nonspecific adsorption-based FN-G nano-layer formation technique is potentially applicable to other polymer scaffold systems for diverse tissue engineering applications.