[Preparation of Mycoplasma antigens and appropriate swine antisera]

An account is given in this paper of results obtained from development of methods for preparation of mycoplasma antigens and appropriate antisera from swine, as compared to normal swine sera. The exercise had been undertaken with the view to diagnosing mycoplasmosis in swine, on the basis of long-time complement fixation in microvolume. Tests were applied to 5 patterns of vaccination of swine, using antigens from mycoplasma. Benefits and drawbacks are discussed in some detail. Also described are methods for preparation, preservation, and storage of mycoplasma diagnostics which retain their suitability for the above diagnostic approach on the basis of 2-4 years of shelf life.