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An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model
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Reporter cell lines for skin sensitization testing.
1Biosciences, Givaudan Schweiz AG, Ueberlandstrasse 138, 8600, Duebendorf, Switzerland. andreas.natsch@givaudan.com.
Archives of Toxicology
|July 22, 2015
Summary
Reporter cell assays are advancing toxicology by measuring molecular events in skin sensitization. These assays, like KeratinoSens™, offer reproducible data for hazard assessment and integrated testing strategies.
Area of Science:
- Toxicology
- Molecular Biology
- In vitro assays
Background:
- Skin sensitization is an adverse outcome pathway (AOP) involving molecular events.
- New toxicology paradigms integrate data from mechanistic tests targeting AOPs.
- High-throughput screening of cellular events is increasingly available.
Purpose of the Study:
- Review existing and emerging reporter cell assays for skin sensitization.
- Discuss their role within the AOP framework.
- Evaluate their utility in hazard and risk assessment and integrated testing strategies.
Main Methods:
- Review of reporter cell line assays (e.g., KeratinoSens™, THP-G8).
- Analysis of Nrf2 and Interleukin-8 pathway activation.
- Exploration of cellular markers and development of new reporter assays.
Main Results:
- KeratinoSens™ (Nrf2 pathway) is an endorsed cell-based assay for skin sensitization.
- Alternative assays like THP-G8 (Interleukin-8) are under development.
- Potential exists for new assays providing non-redundant information.
Conclusions:
- Reporter cell assays are valuable tools for skin sensitization assessment.
- Integration of these assays enhances toxicological predictions.
- Further development aims to improve assay predictivity.
