Related Experiment Video

Updated: Apr 6, 2026

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
06:53

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Published on: January 18, 2019

14.9K

Comparative Evaluation of Two Methods for Preparative Fractionation of Proteinaceous Subvisible

Björn Boll1,2, Emilien Folzer1,3,2, Christof Finkler1

  • 1Pharma Technical Development (Biologics) Europe, Analytical Development and Quality Control, F. Hoffmann-La Roche Ltd., Grenzacherstrasse 124, 4070, Basel, Switzerland.

Pharmaceutical Research
|July 22, 2015
PubMed
Abstract

Keywords:
particle fractionationparticle sizeprotein aggregationproteinaceous particlessubvisible particles

More Related Videos

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification10:19

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

Published on: August 12, 2021

4.9K
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

24.2K

Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
9.7K
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
9.0K
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
8.3K