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Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
Published on: January 3, 2016
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Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE2RD) for diagnostics.
Fatih Inci1, Chiara Filippini2, Murat Baday3
1Demirci Bio-Acoustic-Microelectromechanical Systems in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Radiology Department, Stanford University School of Medicine, Palo Alto, CA 94304;
Summary
A new nanoplasmonic biosensor (NE(2)RD) offers label-free, multi-analyte detection in clinical samples. This portable platform achieves broad dynamic range and high sensitivity, enabling point-of-care diagnostics.
Area of Science:
- Nanotechnology
- Biosensing
- Medical Diagnostics
Background:
- Biosensing advances offer diagnostic potential but face challenges in creating versatile, label-free platforms for diverse clinical samples.
- Current limitations include lengthy sample processing and narrow dynamic ranges, hindering widespread clinical and home use.
Purpose of the Study:
- To develop a label-free, general sensing platform for detecting multiple biotargets in various clinical specimens.
- To overcome limitations of existing biosensors, including sample processing, dynamic range, and portability.
Main Methods:
- Demonstration of the nanoplasmonic electrical field-enhanced resonating device (NE(2)RD) utilizing an immunodetection assay.
- Label-free detection of spectral color changes via wavelength and extinction intensity shifts in nanoparticles.
- Ultrasensitive measurements achieved through self-assembled gold nanoparticles forming hot spots on a 3D substrate.
Main Results:
- The NE(2)RD platform demonstrated label-free, quantitative, and portable detection of diverse analytes including protein biomarkers, drugs, allergens, bacteria, cells, and viruses.
- Achieved a linear dynamic range five orders of magnitude broader than ELISA, with a sensitivity of 400 fg/mL.
- Successfully processed multiple clinical samples (whole blood, serum, saliva) without preprocessing under varying environmental conditions.
Conclusions:
- The NE(2)RD platform addresses key challenges in biosensing, offering a versatile, sensitive, and portable solution.
- Its broad dynamic range, low detection limit, and ability to handle various samples position it for precision medicine applications.
- Potential for enabling point-of-care, primary care, and home-based diagnostics.
