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Glucose biosensor based on GOx/HRP bienzyme at liquid-crystal/aqueous interface.

Mashooq Khan1, Soo-Young Park1

  • 1Department of Polymer Science and Engineering, School of Applied Chemical Engineering, #1370 Sangyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea.

Journal of Colloid and Interface Science
|July 22, 2015
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Summary

This study developed a novel glucose biosensor using co-immobilized glucose oxidase (GOx) and horseradish peroxidase (HRP) on a specialized copolymer. The transmission electron microscope (TEM) grid biosensor offers sensitive and stable glucose detection in water.

Keywords:
BienzymeBiosensorGlucoseGlucose oxidaseLiquid crystalPeroxidase

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Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Materials Science

Background:

  • Enzyme immobilization is crucial for biosensor development.
  • Liquid crystal-based biosensors offer unique detection mechanisms.
  • Transmission electron microscopy (TEM) grids can be adapted for biosensing applications.

Purpose of the Study:

  • To co-immobilize glucose oxidase (GOx) and horseradish peroxidase (HRP) onto a novel poly(acrylicacid-b-4-cyanobiphenyl-4'-undecylacrylate) (PAA-b-LCP) copolymer.
  • To develop a TEM grid-based glucose biosensor for detecting glucose in aqueous solutions.
  • To optimize enzyme loading and investigate biosensor performance characteristics.

Main Methods:

  • Co-immobilization of GOx and HRP onto the PAA block of PAA-b-LCP in water.
  • Anchoring of the PAA-b-LCP copolymer within a 4-cyano-4'-pentylbiphenyl (5CB) liquid crystal matrix on a TEM grid.
  • Detection of glucose via changes in 5CB liquid crystal orientation (planar to homeotropic) observed using polarized optical microscopy.
  • Optimization of GOx/HRP molar ratio and assessment of enzyme activity and stability.

Main Results:

  • The TEM grid biosensor successfully detected glucose concentrations as low as 0.02 mM.
  • An optimal GOx/HRP molar ratio of 3/1 was determined for enhanced performance.
  • The biosensor demonstrated good enzyme sensitivity, stability, and reusability.
  • Selective glucose detection was achieved through the liquid crystal orientation change.

Conclusions:

  • A novel and effective TEM grid-based glucose biosensor was successfully developed.
  • The biosensor utilizes enzyme-liquid crystal interactions for sensitive and selective glucose detection.
  • This approach offers a promising new method for glucose monitoring with potential for practical applications.