Related Experiment Video
Updated: Aug 4, 2026

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
Published on: April 2, 2016
CEMAsuite: open source degenerate PCR primer design
Courtney E Lane1, Daniel Hulgan1, Kelly O'Quinn1
1Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Unlabelled:
The codon-equivalent multiple alignment suite begins conservational analysis for polymerase chain reaction primer design at the protein level, allowing the user to design consensus primers capable of detecting homologous coding sequences even when low-to-moderate sequence information is available. This package also condenses the wealth of information associated with multiple sequence alignments and presents them in an intuitive manner, allowing the user to quickly and effectively address degenerate primer design considerations.
Availability And Implementation:
https://sourceforge.net/projects/cemasuite/.
Contact:
benton@lsu.edu or cemasuite@gmail.com
Supplementary Information:
Supplementary data are available at Bioinformatics online.

