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Related Experiment Videos

Chemosensitivity testing in a tumor acquisition, propagation, and preservation program.

W M Lewko1, S K Nayak, W J Hubbard

  • 1Biotherapeutics, Inc., Franklin, TN 37065-1676.

Molecular Biotherapy
|January 1, 1989
PubMed
Summary

Chemosensitivity assays using propagated tumor cells (xenografts, cell lines) show promise for cancer biotherapy when biopsy tissue is limited. While insensitivity predicts resistance, sensitivity prediction requires further validation.

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Area of Science:

  • Oncology
  • Cancer Research
  • Biotherapy

Background:

  • Chemosensitivity assays are crucial for cancer biotherapy.
  • Tumor acquisition, propagation, and preservation programs utilize these assays.
  • Alternative specimens like cultured cells or xenografts are used when biopsy material is insufficient.

Purpose of the Study:

  • To evaluate the utility of chemosensitivity assays using various tumor specimen types.
  • To compare the success rates of chemosensitivity assays across different specimen sources.
  • To assess the predictive value of chemosensitivity assays against clinical outcomes.

Main Methods:

  • Utilized an adhesive tumor cell culture system for chemosensitivity testing.
  • Assayed 96 specimens out of 154 submitted, including primary biopsies, metastases, xenografts, and cell lines.

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  • Compared assay results with clinical drug treatment outcomes for 18 patients.

    • Main Results:

    • Assay success rates varied: 55% for biopsies, 67% for metastases, 69% for xenografts, and 70% for cell lines.
    • No significant differences in drug sensitivity were observed between tumor cells from biopsies, xenografts, or tissue culture.
    • Assays indicating insensitivity predicted resistance, but assays indicating sensitivity did not reliably predict sensitivity.

    Conclusions:

    • Propagated tumor materials (xenografts, cell lines) are viable alternatives when biopsy tissue is unavailable for chemosensitivity testing.
    • Chemosensitivity assays show potential in predicting drug resistance.
    • Further research is needed to improve the predictive accuracy of chemosensitivity assays for drug sensitivity.