Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiomyocyte-derived BDNF restricts cardiac fibrosis by decreasing the activity of the TGF-β/Smad2/3 pathway and increasing Smad7 expression.

Frontiers in cell and developmental biology·2026
Same author

Permissive and instructive <i>Hox</i> codes govern limb positioning.

eLife·2026
Same author

The impact of COVID-19 on the treatment and prognosis of cancer patients.

International journal of surgery (London, England)·2026
Same author

A watercress drink increases detoxification of common environmental carcinogens such as acrolein and benzene: results of a randomized clinical trial.

Carcinogenesis·2026
Same author

Microwave-alkali co-activated persulfate enables minute-scale fertilization of food waste with high fulvic-like acid yield.

Nature communications·2026
Same author

Fabrication of Multifunctional Starch Mulch Film for Remediation of Pb(II)/Cr(VI) and Slow Release of NH<sub>4</sub><sup>+</sup> in Soil.

Langmuir : the ACS journal of surfaces and colloids·2025
Same journal

Epidemiological characteristics of amebiasis in Japan from 2001 to 2022.

PloS one·2026
Same journal

Longitudinal associations of academic stress with eating related patterns, nutrition, somatic indicators, and depressive symptoms in university students: A study protocol.

PloS one·2026
Same journal

Pollution removal efficiency enhancement by agricultural biomass additions in constructed wetlands: A framework integrating meta-analysis with explainable machine learning.

PloS one·2026
Same journal

Insulation failure mapping on power transformer bushing using FRA and electrostatic simulation.

PloS one·2026
Same journal

Enhancing medical Q&A systems with multimodal knowledge graphs and dual-layer attention mechanisms.

PloS one·2026
Same journal

UAMP: Consistent video object segmentation with uncertainty-aware memory propagation.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Apr 6, 2026

Author Spotlight: Cardiac Cell Transgenesis for Rapid Gene Screening
05:06

Author Spotlight: Cardiac Cell Transgenesis for Rapid Gene Screening

Published on: May 24, 2024

1.3K

Integrative Analysis of the Developing Postnatal Mouse Heart Transcriptome.

Jingyi Gan1, Hans-Joachim Sonntag2, Mei Kuen Tang1

  • 1Stem Cell and Regeneration Thematic Research Programme, School of Biomedical Sciences, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, Hong Kong.

Plos One
|July 23, 2015
PubMed
Summary
This summary is machine-generated.

Postnatal mouse heart development involves cardiomyocyte cell cycle exit, regulated by gene expression changes. This study identifies key molecular players that could potentially reactivate cardiomyocyte proliferation in damaged hearts.

More Related Videos

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
06:27

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Published on: April 17, 2019

8.2K
Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
07:30

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

8.7K

Related Experiment Videos

Last Updated: Apr 6, 2026

Author Spotlight: Cardiac Cell Transgenesis for Rapid Gene Screening
05:06

Author Spotlight: Cardiac Cell Transgenesis for Rapid Gene Screening

Published on: May 24, 2024

1.3K
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
06:27

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Published on: April 17, 2019

8.2K
Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
07:30

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

8.7K

Area of Science:

  • Cardiovascular Biology
  • Developmental Biology
  • Molecular Biology

Background:

  • Mammalian cardiomyocytes proliferate extensively during fetal development and briefly after birth.
  • Cardiomyocytes subsequently enter a quiescent state, but the molecular mechanisms driving this growth arrest remain incompletely understood.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying cardiomyocyte cell cycle exit during postnatal heart development.
  • To identify potential regulators for inducing cardiomyocyte proliferation in the context of cardiac injury.

Main Methods:

  • Comparative transcriptomic analysis (microarray) of postnatal mouse hearts at 2 days (proliferating cardiomyocytes) and 13 days (growth-arrested cardiomyocytes).
  • Bioinformatic analyses including Functional annotation, Gene Ontology (GO), KEGG pathway, and Gene Set Enrichment Analysis (GSEA).
  • Ingenuity Pathway Analysis (IPA) and Regulator Effects network analysis.
  • Validation of differentially expressed genes and key pathway components using RT-qPCR.

Main Results:

  • Down-regulation of cell cycle, DNA replication, chromosome segregation, and microtubule cytoskeleton-associated genes.
  • Up-regulation of immune response, extracellular matrix, cell differentiation, and cell membrane-associated genes.
  • Identification of GATA4, MYH7, and IGF1R as key drivers in gene networks, and TASP1, TOB1, C1orf61, AIF1, ROCK1, TFF2, and miR503-5p as potential inhibitors of cardiomyocyte proliferation.

Conclusions:

  • The study provides novel insights into the transcriptional regulation governing cardiomyocyte cell cycle exit during postnatal development.
  • Identified regulators offer potential therapeutic targets for stimulating cardiomyocyte regeneration in infarcted hearts.