Tyrosinase conjugated reduced graphene oxide based biointerface for bisphenol A sensor.

Area of Science:

• Materials Science
• Electrochemistry
• Environmental Science

Background:

• Bisphenol A (BPA) is an endocrine-disrupting chemical found in many consumer products.
• Conventional methods for BPA detection are often time-consuming and lack sensitivity.
• Development of rapid, sensitive, and selective biosensors is crucial for environmental and health monitoring.

Purpose of the Study:

• To fabricate a highly sensitive electrochemical biosensor for BPA detection.
• To utilize the synergistic properties of reduced graphene oxide (rGO) and chitosan (Cn) for enhanced sensor performance.
• To evaluate the biosensor's sensitivity, selectivity, and applicability in real samples.

Main Methods:

• Fabrication of a reduced graphene oxide (rGO)-chitosan (Cn) nanocomposite.
• Characterization of the nanocomposite using electron microscopy, X-ray diffraction, and FTIR.
• Electrochemical detection of BPA using cyclic voltammetry and amperometry.
• Testing the biosensor's performance with standard BPA solutions and real water samples.

Main Results:

• The rGO-Cn nanocomposite exhibited superior conductivity and a larger effective surface area.
• The fabricated biosensor demonstrated high sensitivity towards BPA, with a limit of detection of 0.74 nM.
• Detection time was significantly reduced to 10 seconds compared to conventional methods.
• The biosensor showed a wide linearity range (0.01–50 µM) and good selectivity for BPA.
• Successful quantification of BPA in packaged drinking water samples was achieved.

Conclusions:

• The rGO-Cn nanocomposite is a promising electrode material for developing highly sensitive electrochemical biosensors.
• This biosensor offers a rapid, sensitive, and selective method for BPA detection.
• The developed biosensor has potential applications in environmental monitoring and food safety analysis.
• The rGO-Cn nanocomposite platform may be extended for the detection of other estrogenic substrates.