Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engineered Bacterial Ligand From Streptococcus pneumoniae on Macroporous Resins for Selective Affinity Capture of Secretory IgA.

Biotechnology and bioengineering·2026

Same author

Structural characterization of the Cu(II)-NTA spin label on α-helices by X-ray crystallography and electron paramagnetic resonance.

Structure (London, England : 1993)·2026

Same author

Tetravalent antibodies are more potent and efficacious erythropoiesis-stimulating agents than erythropoietin in vivo.

Protein science : a publication of the Protein Society·2026

Same author

Allostery Links hACE2 Binding, Pan-variant Neutralization and Helical Extension in the SARS-CoV-2 Spike Protein.

Journal of molecular biology·2025

Same author

Anti-SARS-CoV-2 antibodies in a nasal spray efficiently block viral transmission between ferrets.

iScience·2024

Same author

Artesunate treats obesity in male mice and non-human primates through GDF15/GFRAL signalling axis.

Nature communications·2024

Search research articles

Related Experiment Video

Updated: Apr 6, 2026

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent.

Kristiina Karro¹, Tiiu Männik, Andres Männik

¹Icosagen AS, Icosagen Group, Eerika tee 1, Ossu Village, Ulenurme Parish, 61713, Tartumaa, Estonia.

Methods in Molecular Biology (Clifton, N.J.)

|July 24, 2015

Summary

We developed NickFect51, a stearylated cell-penetrating peptide (CPP), for efficient DNA vector delivery into various animal cells. This universal method benefits gene therapy, cell biology, and protein production research.

More Related Videos

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Engineering Cell-permeable Protein

Engineering Cell-permeable Protein

Published on: December 28, 2009

Related Experiment Videos

Last Updated: Apr 6, 2026

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Engineering Cell-permeable Protein

Engineering Cell-permeable Protein

Published on: December 28, 2009

Area of Science:

Molecular Biology
Biotechnology
Cell Biology

Background:

Efficient gene delivery into cells is crucial for therapeutic applications, research, and protein production.
Current transfection methods can be limited in scope or convenience.

Purpose of the Study:

To develop a universal and efficient method for DNA vector delivery into diverse animal cell types.
To utilize a stearylated cell-penetrating peptide (CPP) for enhanced transfection.

Main Methods:

Development and application of a novel stearylated CPP, termed NickFect51.
Testing transfection efficiency across various cell lines (mammalian, avian, insect).
Evaluation of suitability for protein production cell lines in serum-free conditions.

Main Results:

NickFect51 demonstrated effective DNA vector delivery into mammalian, avian, and insect cells.
The method proved efficient for common research cell lines.
Particular suitability was observed for protein production cell lines grown in chemically defined serum-free media.

Conclusions:

The stearylated CPP, NickFect51, offers an effective, universal, and convenient solution for gene delivery.
This method supports advancements in nucleic acid therapeutics, molecular studies, and recombinant protein production.