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Updated: Apr 6, 2026
How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow
Published on: November 11, 2010
Fiber-based laser speckle imaging for the detection of pulsatile flow
Caitlin Regan1,2, Bruce Y Yang1, Kent C Mayzel1,2
1Beckman Laser Institute and Medical Clinic, University of California, Irvine, California, 92612.
Background And Objective:
In endodontics, a major diagnostic challenge is the accurate assessment of pulp status. In this study, we designed and characterized a fiber-based laser speckle imaging system to study pulsatile blood flow in the tooth.
Study Design/Materials And Methods:
To take transilluminated laser speckle images of the teeth, we built a custom fiber-based probe. To assess our ability to detect changes in pulsatile flow, we performed in vitro and preliminary in vivo tests on tissue-simulating phantoms and human teeth. We imaged flow of intralipid in a glass microchannel at simulated heart rates ranging from 40 beats/minute (bpm) to 120 bpm (0.67-2.00 Hz). We also collected in vivo data from the upper front incisors of healthy subjects. From the measured raw speckle data, we calculated temporal speckle contrast versus time. With frequency-domain analysis, we identified the frequency components of the contrast waveforms.
Results:
With our approach, we observed in vitro the presence of pulsatile flow at different simulated heart rates. We characterized simulated heart rate with an accuracy of and >98%. In the in vivo proof-of-principle experiment, we measured heart rates of 69, 90, and 57 bpm, which agreed with measurements of subject heart rate taken with a wearable, commercial pulse oximeter.
Conclusions:
We designed, built, and tested the performance of a dental imaging probe. Data from in vitro and in -vivo tests strongly suggest that this probe can detect the presence of pulsatile flow. LSI may enable endodontists to noninvasively assess pulpal vitality via direct measurement of blood flow.
